Cornea Suturing Wetlab
· Understand the physics of corneal wound/incision closure
· Create and close linear and stellate corneal incisions
· Pig/goat eyes
· Supersharp blades
· Fine non-locking needle holders
· 10-0 nylon suture
· Colibri or straight 0.12 forceps
· Vannas scissors
· Tying forceps
· Please read the following:
o Macsai chapter on physics of wound closure
o Macsai chapter on traumatic laceration repair
o Krachmer section on corneal laceration repair
1. Inject some BSS into the anterior chamber of the pig/goat eye to reform the anterior chamber to physiologic turgor.
2. Use a blade to create a linear partial thickness incision in the cornea.
3. Close this incision with 10-0 nylon sutures in an interrupted fashion.
4. Repeat process with a full thickness linear incision as well as a stellate (Mercedes sign) full thickness incision.
5. Reform the anterior chamber with BSS to confirm that wound is free of leakage.