Pizza at 5:30pm in EEI 150.
Also, please remember to double bag any waste into the red Biohazard bag.
Then place in cardboard Biohazard box. Put in hallway outside the door of the wetlab.
Call Maintenance at 412-647-3370 for pick up.
Learning Objectives for PKP
- Understand the principles of wound creation and closure with penetrating keratoplasties
Materials
- Pig/goat eyes
- Supersharp blades
- Viscoelastic
- BSS
- Fine non-locking needle holders
- 10-0 nylon suture
- Colibri or straight 0.12 or 0.3 forceps
- Vannas scissors
- Tying forceps
- Vacuum trephine
- Left and right corneal scissors
Preparation
- Please read the following:
- Macsai chapter on corneal suturing
- Krachmer section on penetrating keratoplasty
- Please watch the following videos:
- Trephination and corneal excision in PKP (will need to create free account if don’t already have one):
- Full surgical video on PKP
- https://www.youtube.com/watch?v=lxLmOPWL1bY
Procedure
- Inject some BSS into the anterior chamber of the pig/goat eye to reform the anterior chamber to physiologic turgor (makes for easier paracentesis incision)
- Create a paracentesis incision with a 15-degree blade
- Fill anterior chamber with viscoelastic
- Trephine the cornea with the vacuum trephine.
- Excise corneal button with left and right corneal scissors
- Resuture corneal button back in PKP fashion
Objectives for Ambio-Disk / AMT membrane
- Review indications for amniotic membrane transplantation
- Become familiar with and demonstrate the technique of amniotic membrane transplantation to close a conjunctival defect
- Become familiar with and demonstrate the technique of amniotic membrane transplantation to repair corneal pathology
- Review post-operative care after AMT
- Discuss complications
Instruments and Supplies
- Needle driver
- 0.12 forceps with tying platform
- Marking pen
- Crescent blade
- Wescott scissors
- Vannas scissors
- Tying forceps
- Amniotic membrane
- 8-0 Vicryl suture
- 10-0 Nylon suture
- Fibrin glue
